To solve the problems of large reagent consumption and aerosol contamination in tube-based detection of traditional real-time fluorescent quantitative PCR instruments, a disc-shaped microfluidic chip integrated with sealed valves was proposed. Based on the mechanisms of nucleic acid amplification reaction, the intrinsic requirements for high temperature and airtightness were analyzed, and a multiphysics?field coupling model was constructed to optimize the key structural parameters of microchannels and valve groups. Combining simulations and physical experiments, we compared the fluid burst pressure of valve units under different parameters to validate the optimal valve structure. Furthermore, reliability evaluations of the sealed valves were completed by conducting high-temperature thermal cycling sealing tests using a custom-built fixture. By establishing a nucleic acid amplification experimental system, amplification efficiency was evaluated on various samples using SYBR Green fluorescence chemistry, alongside parallel controls on a traditional tube-based platform. The results show that the optimized chip has excellent performance: the relative weight change rate after sealing is only 0.379% (vs. 98.588% before sealing); the coefficients of variation of the experimental and control groups are 4.73% and 0.27% respectively, meeting the precision requirements. The detection results are highly consistent with the traditional platform (R2=0.995), realizing accurate detection of target nucleic acids. Integrating 16 independent detection units for synchronous parallel detection, the chip provides a novel solution for highthroughput rapid nucleic acid screening and has broad clinical application prospects. Keywords:disc-shaped microfluidic chip; real-time fluorescent quantitative PCR; capillary valve; sealing valve