In order to realize the accurate measurement and tracking of UAV LOS stabilization system, a LOS stabilization controller based on classical PID and fuzzy control is designed. There are a lot of uncertainties in the process of PID parameter tuning in practical engineering. In order to realize the on-line tuning of PID parameters, this paper combines fuzzy control algorithm with classical PID control, and constructs a parameter self-tuning fuzzy PID controller, which realizes the correction of PID controller. In MATLAB Fuzzy Toolbox and Simulink, comparing PID with parameter self-tuning fuzzy PID, the step response curves of parameter self-tuning fuzzy PID controller under no disturbance and 10 Hz sinusoidal disturbance show that fuzzy PID has shorter response time and smaller isolation degree than fuzzy control and PID control, and the fuzzy PID error occurs in the sinusoidal response curve of the system with input from 1~10 Hz. The difference is the smallest and the control effect is the best. Thus, the self tuning PID fuzzy controller has good robustness and control performance in LOS stabilization.