In order to solve the problems of low sensitivity and narrow linear range of fluorescence immunoassay, an immune analyzer based on immunochromatography assay and time-resolved fluorescence microparticles was designed. In the system, the detection object is the fluorescent reagent card, the marker of which is the time-resolved fluorescence microparticles. The excitation light is monochromatic LED (340 nm). The fluorescence emitted by the fluorescence microparticles on the T line and C line of the reagent card is detected by photodiode. The concentration of the measured substance can be calculated using the fluorescence value together with the standard concentration curve. The results show that the immune analyzer shows up good linearity in the range of 0.5~200mg/mL, with linear correlation coefficient R2=0.9988. The precision of the immune analyzer is also pretty good since the instrument CV is generally less than 1%, and the inter-instrument CV is no more than 2.5%. The merit of simple operation and fast detection allows the immune analyzer to be applied in a wide range of fields, such as the POCT, drug detection, food safety detection and so on.