PCI bus is widely used in modern electronic test and measurement fields. However, current testing facilities based on the PCI bus are mainly Target devices of PCI bus, which greatly limits the performance of testing facilities. This paper, firstly, presents the working principle in Initiator mode of PCI bus and then realizes the function of testing facilities worked in Initiator mode of PCI bus by the combination of PLX PCI9054 chip and FPGA. The design can effectively improve the realtime performance among testing facilities on data interaction, and can be used as connection between testing facilities and dedicated storage device. At last, the paper presents examples between devices which are worked in Initiator mode of PCI bus and fully verify the feasibility of the design.